The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve.
Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2 -Delta Delta C T method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments.
CFX Manager Protocol and Plate Setup
The purpose of this report is to present the derivation, assumptions, and applications of the 2 -Delta Delta C T method. In addition, we present the derivation and applications of two variations of the 2 -Delta Delta C T method that may be useful in the analysis of real-time, quantitative PCR data.
National Center for Biotechnology Information , U. Didn't get the message?
Find out why Add to Clipboard. Add to Collections.
Order articles. Fetching bibliography My Bibliography Add to Bibliography. Generate a file for use with external citation management software.
Create File. Abstract The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification.